Validation of genetic test for FSF – Samples Needed!!!!!

Test on November 7th (next Sunday) in Clayton, CA

Calling all Shar-Pei (papered or not)

All the years of research has finally produced results. They have located the mutated gene that causes Shar-Pei Fever. But they have to run a validation study to confirm their findings.



Dr. Linda Tintle, DVM has put out an request for additional samples from older than 5-years-old Shar-Pei. At this point, a pedigree is not as important, but pure bred is. Her request and instructions follow.



To facilitate gathering of the much needed samples, the Chinese Shar-Pei Club of Northern California is holding a blood draw for this test on November 7th (next Sunday) in Clayton, CA. The Vet techs doing the draw have 15 to 30 yrs experience with Shar-Pei. There is no cost to participants and CSPCNC will be shipping all the samples together. If you are interested in having your Shar-Pei participate in this draw please contact the club at cspcnc@yahoo.com. Or if you can't make this date, contact us and we can make other arrangements for late next week.



These additional research samples are being funded by the Chinese Shar-Pei Charitable Trust ( http://www.cspcharitabletrust.org/Home.cfm.)



If you submit samples on your own, please have your vet follow the instructions to the letter. In layman's terms, 5ml of both the purple top (EDTA whole blood) and the red top (serum) vials need to be used. Do not freeze sample but keep on ice and shipped in ice overnight. No deliveries can be received on a Sat or Sun. The instructions are all listed on the questionnaire, link below. And make sure to include a "complete medical file." No shaving of the dog is needed, just one little poke. In a minute or two it is all over!



Please find it in your heart to participate in this ground breaking research. My 11-year-old has participated in this and all the other requests over the years.



Current research information supporting these findings are in peer review and should be published next spring or summer. When it is published I will make sure the information is published here. Individual results of this validation test will be available, privately, to each of the participants upon written request at no charge. When this test is validated, the test will be available to all Shar-Pei, for a fee.



Deanna Liskey, CSPR volunteer & CSPCNC President



XX Permission to Cross-Post (Please!) XX




Validation of genetic test for FSF – Samples Needed.




We have received a very large number of samples at the Broad Institute and are grateful for the response. But we have received too few from the most valuable groups:

1) Shar-Pei with a history of FSF and good medical records

2) Shar-Pei with no FSF over 5 yrs of age.




Many samples were received of EDTA whole blood only without accompanying serum samples and this restricts their value to the study. I realize that some of this occurred because the Broad Institute’s generic release form stated 5 ml of EDTA whole blood while the Questionnaire for the Shar-Pei study that followed that release requested 5 ml EDTA whole blood AND serum from 5 ml blood. Many samples were unfortunately received without serum.




In order to get sufficient samples for validating the test, I am asking the Shar-Pei community to consider obtaining blood and serum samples from their older Shar-Pei and sending them to the Broad.




AKC#s and pedigrees are helpful but it is much more important at this juncture that we get samples from older dogs ( > 5 yrs ) with good medical records. If you did not participate because your older Shar-Pei is not registered or has an unknown pedigree, please reconsider contributing by sending samples now.




We have many samples from the dogs at the CSPCA National Specialty but, as you would expect, many of these dogs were less than 5 yrs of age.




Information and downloadable PDF of release and questionnaire to accompany samples can be found on my website here:




http://www.wvc.vetsuite.com/Templates/ContentPages/More_Info/ClinicContentPage.aspx?guid=96c77dba-9a72-4402-8d7e-fe2150621058